assay curve

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• Standard curve — A standard curve is a quantitative research tool, a method of plotting assay data that is used to determine the concentration of a substance, particularly proteins and DNA. It can be used in many biological experimentsThe assay is first performed …   Wikipedia

• Enzyme assay — Beckman DU640 UV/Vis spectrophotometer. Enzyme assays are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and enzyme inhibition. Contents …   Wikipedia

• Bradford protein assay — The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. It is subjective, i.e. dependent on the amino acid composition of the measured protein. PrincipleThe Bradford assay, a… …   Wikipedia

• Clonogenic assay — A clonogenic assay is a microbiology technique for studying the effectiveness of specific agents on the survival and proliferation of cells. It is frequently used in cancer research laboratories to determine the effect of drugs or radiation on… …   Wikipedia

• Calibration curve — In analytical chemistry, a calibration curve is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration.The calibration curve is a plot of …   Wikipedia

• MTT assay — is a laboratory test and a standard colorimetric assay (an assay which measures changes in color) for measuring the activity of enzymes that reduce MTT to formazan, giving a purple color. This mostly happens in mitochondria, and as such it is in… …   Wikipedia

• Filter binding assay — In biochemistry, one of the ways to learn about an interaction between two molecules is to determine the binding constant, which is a number that describes the ratio of unbound and bound molecules. This information reveals the affinity between… …   Wikipedia

• analysis — /euh nal euh sis/, n., pl. analyses / seez /. 1. the separating of any material or abstract entity into its constituent elements (opposed to synthesis). 2. this process as a method of studying the nature of something or of determining its… …   Universalium

• Enzyme kinetics — is the study of the chemical reactions that are catalysed by enzymes, with a focus on their reaction rates. The study of an enzyme s kinetics reveals the catalytic mechanism of this enzyme, its role in metabolism, how its activity is controlled,… …   Wikipedia

• Bisulfite sequencing — Figure 1: Outline of bisulfite conversion of sample sequence of genomic DNA. Nucleotides in blue are unmethylated cytosines converted to uracils by bisulfite, while red nucleotides are 5 methylcytosines resistant to conversion …   Wikipedia

• Microscale thermophoresis — (MST) is a technology for the analysis of biomolecules. Microscale Thermophoresis is the directed movement of particles in a microscopic temperature gradient. Any change of the hydration shell of biomolecules due to changes in their… …   Wikipedia